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Massie stained gel of purified GST-Roc proteins used in the ligase assays. Bracket indicates GST-Roc proteins. Asterisk indicates GST. Lower bands are degradation products. B, All Drosophila Roc proteins and chimeras were assessed for ligase activity in a substrate free assay. Briefly, 250 ng of Roc protein (or control GST) were added to a ubiquitin ligase mixture containing UbcH5, ubiquitin, and
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